Toxin Detection by Cell Culture Neutralization Assay [CYT] and Toxin based EIA [Tox EIA] among Recurrent Episodes of CDI Diagnosed by PCR
نویسندگان
چکیده
1287. Differences in Clinical Characteristics and Outcomes of Patients with Community-Onset Clostridium difficile Infection who Tested Positive by EIA Compared with NAAT through a Two-Step Algorithm Erin C. Phipps, DVM, MPH; Nicole Kenslow, MPH; Ioan Belovarski, MD, MPH and Emily B. Hancock, MS; New Mexico Emerging Infections Program, University of New Mexico, Albuquerque, New Mexico, University of New Mexico School of Medicine, Albuquerque, New Mexico
منابع مشابه
Rapid enzyme immunoassay for determination of toxigenicity among clinical isolates of corynebacteria.
A rapid enzyme immunoassay (EIA) was developed for the phenotypic detection of diphtheria toxin among clinical isolates of corynebacteria. The assay uses equine polyclonal antitoxin as the capture antibody and an alkaline phosphatase-labeled monoclonal antibody, specific for fragment A of the toxin molecule, as the detecting antibody. The assay is rapid, sensitive, and specific: a final result ...
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Clostridium difficile infection (CDI) caused by toxigenic strains of C. difficile is primarily a nosocomial infection with increasing prevalence. Stool specimens are typically collected in Cary-Blair transport medium to maximize culture-based detection of common stool pathogens. The goal of this study was to establish an analytically accurate and efficient algorithm for the detection of CDI in ...
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Stool culture for Clostridium difficile, while necessary for strain typing and antimicrobial surveillance, cannot determine toxin production. We prospectively tested in triplicate 91 C difficile cultured isolates for toxin production by 2 enzyme immunoassays (EIAs) (Meridian Premier Toxins A&B, Meridian Bioscience, Cincinnati, OH; and TechLab Tox A/B II, TechLab, Blacksburg, VA) and cytotoxin n...
متن کاملCharacteristics of Clostridium difficile infection in patients with discordant diagnostic test results.
BACKGROUND Clinical features of Clostridium difficile infection (CDI) cases diagnosed by detection of polymerase chain reaction (PCR), with negative toxin enzyme immunoassay results (EIA) have not been fully elucidated. The purpose of this study was to determine the magnitude of CDI patients who had negative EIA toxin determinations but positive PCR tests, and their differences in clinical pres...
متن کاملImpact of the type of diagnostic assay on Clostridium difficile infection and complication rates in a mandatory reporting program.
BACKGROUND Most Clostridium difficile infection (CDI) surveillance programs neither specify the diagnostic method to be used nor stratify rates accordingly. We assessed the difference in healthcare-associated CDI (HA-CDI) incidence and complication rates obtained by 2 validated diagnostic methods. METHODS This was a prospective cohort study of patients for whom a C. difficile test was ordered...
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عنوان ژورنال:
دوره 4 شماره
صفحات -
تاریخ انتشار 2017